Monday, 27 September 2010
Multichannel Pipettes - The easy way to increase productivity
Minimise Pipetting Steps with Multichannel Pipettes
Most experimental work starts in individual tubes for which single channel pipettes are ideal. Once protocols have been established the next step is to scale up the research and run larger scale pilot experiments or assay runs, often performed in 96 well microtitre plates. Utilising single channel pipettes at this stage can be extremely time consuming and highly repetitive.
How many pipetting steps?
Have you ever thought about how many pipetting steps it takes to set up a 96 well PCR plate? For example, using a manual single channel pipette it can take as many as 672 individual steps (7 reagents x 96 wells). This amount of pipetting, even with an ergonomically designed single channel pipette can cause hand fatigue and increased risk of RSI, even with correct pipetting technique. If your hands are tired, errors can be introduced that could have detrimental effects on accuracy and precision. This may result in the need to run the plate again, not only wasting valuable reagents but also decreasing productivity and so reducing the advantages of using the 96 well plates in the first place.
Microtitre plates are the reason why multichannel pipettes were designed. A manual 8-channel pipette can fill an equivalent 96 well PCR plate in only 84 pipetting steps. A manual 12-channel pipette can further reduce this to only 56 pipetting steps. These reductions have a significant impact on time savings, reducing the risks of RSI, maintaining the accuracy of pipetting and improving laboratory productivity.
Electronic multichannel pipettes decrease the time taken to fill a 96 well plate, significantly increasing throughput. Tests were carried out using a RAININ Electronic EDP3 8-channel pipette, 1200µl in pipet mode and multi-dispense, autopace mode to fill a 96 well assay plate. The pipette filled the plate, on average, in 67 seconds for the Pipette mode and only 12.6 seconds in Multi-dispense (average time taken over 5 plates).
An essential element of efficient multichannel pipetting is the tips used and their fit. Many pipettes on the market require excessive force to apply tips across all channels and/or time consuming manual adjustments before use. These requirements lead to inconsistent loading of the pipette tips, which is not desirable and can detrimentally effect productivity. Therefore care should be taken when choosing which tips to use with your multichannel pipette to ensure that the benefits of the pipette are not being hampered by ill fitting tips.
Which multichannel pipette? The RAININ Lite Touch Tip Ejection (LTS) range of Electronic and Manual multichannel pipettes are the perfect solution where productivity and RSI is a major concern. The pipettes load tips in as little as 2 seconds. Traditional multichannels can require up to 30 seconds. Researchers often resort to “rocking” the manifold over the tip rack, then hand-tightening each tip in an effort to secure a proper seal. These tedious methods can result in loss of productivity and possible contamination. Poorly fitting tips cause inconsistent loading. Samples and reagents are wasted, and in many cases, work must be re-done. With LTS, you can load tips quickly with confidence and reliability. Simultaneous tip ejection is easy, requiring just a light press of the tip ejection button.
• multi dispense
• standard pipetting
• 3000 pipetting cycles can be achieved in only one charge.
The perfect tips for multichannel pipetting
Anachem offers RAININ pipette tip packaging solutions that enhance the benefits of using multichannel pipettes.
• StableStak and StableRak double 96 racks for easy loading onto either 8 or 12 channel pipettes.
• StableRak is available as a double 96 rack of either aerosol resistant filter tips or standard FinePoint tips.
• Widest tip volume range on the market from 0.1µl up to 1200µl.
• RNase, DNase and Pyrogen free
• Entire units may be autoclaved.
• Enclosed bottom and cover protect tips from contamination in use.
Contact Anachem on 01582 455135 or email@example.com to arrange a deomnstration of the Rainin LTS pipettes to see how you can increase your productivity while reducing your risk of RSI