New PureSpeed™ Protein Tips are the most simple and effective way to purify native and recombinant proteins. PureSpeed™ tips contain a patented packed-resin bed that contains ProA, ProG or IMAC resin within the pipette tip end. This captures, purifies and enriches the protein of interest by bidirectional flow of sample volume through the tip bed. These new innovative tips produce the highest concentration of purified protein, allowing many options for downstream functional assays.
PureSpeed™ Tips are designed for use with Rainin’s E4™XLS™ electronic pipette, which has a dedicated PureSpeed™ mode that enables protein purification to be programmed and controlled in a semi-automated process, simplifying and enhancing the performance of the tip. Processing for up to 12 protein samples, in parallel, is automated in as little as 15 minutes!
FPLC in a Pipette Tip
Capture
PureSpeed’s unique process draws a sample repeatedly over a low dead volume, packed resin bed in the tip’s end, delivering greater saturation, stronger binding kinetics and higher protein concentration than either gravity-fed affinity columns or spin columns.
Purify
Repetitive multiple wash steps remove unbound, nonspecific proteins and contaminants. A unique second wash step optimizes the protein purification process to produce the highest enrichment possible during the final elution step.
Enrich
In the final step, a small volume of low pH elution buffer releases the protein from the resin. With this efficient and low volume system, PureSpeed™ tips produce the highest concentration of purified material of any purification technique.
Minutes not hours!
Rainin’s PureSpeed™ system produces purified protein in as little as 15 minutes. PureSpeed™ tips eliminate the need for additional concentration steps and the PureSpeed™ protocol on Rainin’s E4™XLS™ automates many of the purification steps.
Arrange a trial in your lab or request a PureSpeed™ brochure for a chance to win an E4™XLS™ Electronic Pipette. (Draw closes 30/06/2012).
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